Amino Acid Profile Test - Base Hydrolyzed Amino Acids (Tryptophan) Test
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Price
$225 per analysis
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Turnaround time 12 - 15 Business Days
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Rush Available
Technical data sheet
Print- Method description
Tryptophan is liberated from proteins by alkaline hydrolysis in 4.2N LiOH at 110°C for 22 hours. Hydrolysis is performed under near-vaccum to protect tryptophan from oxygenm and 5-methyl tryptophan is included as an internal standard to account for any losses during hydrolysis. Sample extracts are then diluted and filtered. Analysis is performed on a reversed-phase ultra-performance liquid chromatograph with fluroescence detection (UPLC-FLR).
- Acceptable matrices
Food and Food Ingredients
- Limit of quantitation
0.0001%
- Equipment
HPLC-UV
- Method reference
AOAC 988.15
- Reportable unit
%
- Sample size requirements
50 g
- Information required by submitter
Please supply estimates
- Additional information
Results will be reported as "Tryptophan as Dehydrate" to signify that the reported value for the individual amino acid does not include the molecular weight of water. If you are in need of the hydrated tryptophan value (individual amino acid that includes the molecular weight of one water molecule) please let us know at the time of sample submission.
Common Amino Acid Profile Test - Base Hydrolyzed Amino Acids (Tryptophan) Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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