Beta-Glucan (Mushroom, Yeast) Test
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Price
$285 per analysis
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Turnaround time 12 - 15 Business Days
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Rush Available
Technical data sheet
Print- Method description
Total glucans within a sample are solubilized in ice-cold 12 M H2SO4 and hydrolyzed to near completion in 2 M H2SO4. Remaining glucan fragments are quantitatively hydrolyzed to glucose using a mixture of exo-1,3-βglucanase and β-glucosidase. An additional sample portion undergoes hydrolysis of the α-Glucans to glucose using amyloglucosidase plus a-amylase. The glucose in both sample test portions are measured using GOPOD reagent. β-Glucan is determined by difference between the total glucans and α-glucans.
- Acceptable matrices
Brewers yeast, yeast extract, dried mushrooms, mushrooms
- Unacceptable matrices
Samples containing cereal grain-based Beta Glucan and/or oat-based Beta Glucan
- Limit of quantitation
1%
- Equipment
Waterbath, Spectrophotometer
- Method reference
Megazyme K-YBGL
- Reportable unit
% (w/w)
- Sample size requirements
10 g
- Information required by submitter
Please supply estimates
Common Beta-Glucan (Mushroom, Yeast) Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
Unfortunately, this test only reports Beta-glucan.
Tinture samples must have enough beta-glucan present to be dected by this method. Please view current LOQ/LODs on the assay's tech data sheet.
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