Cholesterol Test
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Price
$150 per analysis
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Turnaround time 7 - 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
Cholestane is used as an internal standard for cholesterol and is added prior to hydrolysis. The components are analyzed using gas chromatography with a capillary column.
- Acceptable matrices
Food and Food Ingredients
- Unacceptable matrices
Concentrates of Vitamin E or forms of Vitamin E such as tocopherols, tocopheryl succinate,tocotrienols, and gums.
- Limit of quantitation
1mg/100g
- Equipment
Gas Chromatograph with Flame Ionization Detection
- Method reference
AOAC 976.26 with modifications
- Reportable unit
mg/100g
- Sample size requirements
5 g
- Information required by submitter
Please supply estimates
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. Lipids are unbound from food samples by acid or base hydrolysis and extracted into mixed ethers. The fatty acid containing compounds are then interesterfied to form fatty acid methyl esters and free sterols. The components are separated and analyzed using a capillary column on a gas chromatograph.
Common Cholesterol Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
The method uncertainty for the cholesterol test is 14.2%.
Food stuff needing cholesterol analysis undergoes an acidic hydrolysis then a lipid extraction step before being derivatized. This derivatization step is crucial as it improves the matrix separation, detection, and resolution providing more accurate quantification from other compounds that closely resembles (phytosterols/fat soluble vitamins) or elutes near the cholesterol peak.
According to 21 CFR 101.62(d), the terms “cholesterol-free”, “low in cholesterol” or similar phrases may be used in labeling provided that “the food contains less than 2mg of cholesterol per reference amount customarily consumed per labeling serving.” Please see the Code of Federal Regulations for more information regarding labeling or health claims.
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