Fiber (Total - Gravimetric) Test
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Price
$200 per analysis
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Turnaround time 7 - 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
Samples (defatted if necessary) are digested with three enzymes (alpha-amylase, protease and amyloglucosidase) to remove starch and protein. Ethanol is added to precipitate soluble dietary fiber. The resulting solution is filtered and the residue is dried and weighed. Protein and ash are determined on the residue. Total dietary fiber (TDF) is calculated as the percent residue minus protein and ash.
- Acceptable matrices
Food and Food Ingredients
- Unacceptable matrices
Baking powder
- Limit of quantitation
Total Dietary Fiber - 0.1%
- Equipment
Standard Gravimetric Analytical Chemistry Equipment
- Method reference
AOAC 991.43 with modifications
- Reportable unit
%
- Sample size requirements
25 g
- Information required by submitter
please supply expected estimates. Indicate matrix type, and indicate if samples are high in fat (>5% fat). Indicate whether the sample contains stevia, fructans (inulin or fructooligosaccharides (FOS)), psyllium, flax, and gum (i.e. guar gum)
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate #2769.01. Dietary Fiber is the portion of the carbohydrates which is not digested in the human gut. It reports the sum of both soluble and insoluble components. These methods are applicable to all food products, however samples containing psyllium or fructans should be submitted under specific test codes. High-fat (>5%) samples require special handling.
Is this the most suitable fiber test for evaluating my product?
Find your fiber source(s) on the chart and use the alpha reference to see how it aligns with each method. If the alpha reference is fully inside the method circle, that method is optimal for your fiber source(s). If it's outside or touching the circle's edge, the method won't fully capture the fiber. Remember to check applicable labeling regulations for allowed fiber sources.
- AOAC Method 2001.03
AOAC Method 991.43 - AOAC Method 2017.16
AOAC Method 2022.01 - AOAC Method 985.29
AOAC Method 991.43 - AOAC Method 2009.01
AOAC Method 2011.25
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Cellulose | Beta-Glucan | Psyllium | Gums | Lignin -
Galacto-oligosaccharides-Raffinose(GOS)/Stachyose -
Galactomannan | Arabinoxylan | Glucomannan -
Resistant Starch RS4 -
Resistant Starch RS2 & RS3 -
Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO) -
Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF) -
Fructans (Inulin, FOS)
- Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
- Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
- Galactomannan | Arabinoxylan | Glucomannan
- Resistant Starch RS4
- Resistant Starch RS2 & RS3
- Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
- Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
- Fructans (Inulin, FOS)
- Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
- Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
- Galactomannan | Arabinoxylan | Glucomannan
- Resistant Starch RS4
- Resistant Starch RS2 & RS3
- Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
- Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
- Fructans (Inulin, FOS)
- Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
- Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
- Galactomannan | Arabinoxylan | Glucomannan
- Resistant Starch RS4
- Resistant Starch RS2 & RS3
- Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
- Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
- Fructans (Inulin, FOS)
Common Fiber (Total - Gravimetric) Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
The Total Gravimetric test does not differentiate between the soluble and insoluble fiber. This test will only measure the total fiber content, which is the sum of the insoluble and soluble fibers.
Fiber testing has several procedures built into the process to remove compounds that are not fiber. Digestible carbohydrates are removed by enzymatic digestion, fat is removed with a defatting process in solvent, etc.
The testing itself does not account for this potential loss in fiber and the results reflect the sample ‘as-received’ by Medallion. It is important to be aware of the regulations for fiber testing, and to be aware of specialized ingredients that may degrade during processing and determine a corrective action. For example, additional fiber ingredients may need to be added to the recipe to account for this loss, or alternative baking steps may need to be considered to ensure the product label is compliant and accurate.
Our food testing experts are here to help.
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