Vitamin B9 (Folic Acid - LC/MS) - Test
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Price
$190 per analysis
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Turnaround time 7 - 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
A homogenized sample is weighed and internal standard and phosphate buffer are added. Amylase and protease enzymes are added to further break down the matrix for extraction of the folates. Conjugase is used to de-conjugate the polyglutamate forms of folates to mono-glutamates. The sample is centrifuged, filtered and cleaned using SPE. The folates are analyzed by UPLC-MS/MS. Folic Acid is quantified. Isotopically labeled internal standards are used to control matrix to matrix variation.
- Acceptable matrices
All cereal products, flours, milk and soy-based products, infant formulas and baby food composite, vegetables, fruits, protein powder and shake, vitamin concentrates (up to 2 % folates), peanuts, peanut butter and other food matrices.
- Unacceptable matrices
Gum, encapsulated vitamins, premixes and supplements above 2%
- Limit of quantitation
10µg/100g - 2,000,000 µg/100g (2 % total folates)
- Equipment
High-Performance Liquid Chromatography with Fluorescence Detection
- Method reference
AOAC 2011.06
- Reportable unit
µg/100g
- Sample size requirements
5 g
- Information required by submitter
Please supply estimates
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. This method is not designed for use on vitamin concentrates, and the vitamer 5-methyl tetrahydrofolate will not be quantified.
Common Vitamin B9 (Folic Acid - LC/MS) - Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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