Lipase Activity Test
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Price
$315 per analysis
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Turnaround time 12 - 15 Business Days
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Rush Available
Technical data sheet
Print- Method description
Samples are homogenized in a cold phosphate buffered saline solution (PBS), pH 8.5 and then centrifuged. A protein determination by the Method of Bradford is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction. The enzyme extract is incubated at 37C with a saturating amount of triglyceride that contains sulfhydryl groups substituted for the normal carboxylic acid groups on the fatty acids. As the extracted lipase in the supernatant cleaves the modified fatty acids from the glycerol backbone, the sulfhydryl groups are made available for reaction with a color reagent (DTNB). The color reagant absorbs visible light at 412nm in direct proportion to the number of sulfhydryl groups exposed by the lipase. The appearance of absorbance with time of reaction is monitored kinetically. The initial velocity of the reaction is determined from the reaction rate.
- Acceptable matrices
cereal grains, doughs, pastas, and vegetables
- Limit of quantitation
2783 Units/g
- Equipment
Waterbath, spectrophotometer, centrifuge
- Method reference
BioAssay Systems Lipase Assay Kit (DLPS-100)
- Reportable unit
Units per gram. One unit of enzyme catalyzes the cleavage of 1 µmole of substrate per minute under the assay conditions (pH 8.5).
- Sample size requirements
20 g
- Information required by submitter
Please supply estimates
Common Lipase Activity Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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