Lipoxygenase Activity Test
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Price
$285 per analysis
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Turnaround time 12 - 15 Business Days
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Rush Available
Technical data sheet
Print- Method description
Samples are homogenized and enzymes are extracted into a cold, pH 6.5 buffer. A protein determination by the Method of Bradford is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction. The enzyme extract is mixed with linoleic acid or arachidonic acid. Lipoxygenase activity is measured spectrophotometrically by the rate of formation of the fatty acid hydroperoxide at 234nm.
- Acceptable matrices
cereal grains, doughs, pastas, and vegetables
- Limit of quantitation
1.98 units/gram
- Equipment
Waterbath, spectrophotometer
- Method reference
Axelrod B, Cheesbrough TM, and Laakso S (1981) Lipoxygenase from soybeans, In “Methods in Enzymology”, Vol. 71. (Ed.) JM Lowenstein. Academic Press, New York
Ben-Aziz A, Grossman S, Ascarelli I, and Budowski P (1970) Linoleate oxidation induced by lipoxygenase and heme proteins: a direct spectrophotometric assay. Anal. Biochem. 34:8
- Reportable unit
Units per gram. One unit is defined as the change in absorbance at 234nm per minute from the hydroperoxidation of the cis, cis-1,4 pentadiene containing fatty acid. One unit is equivalent to the oxidation of 0.12 micromoles of linoleic acid.
- Sample size requirements
20 g
- Information required by submitter
Please supply estimates
Common Lipoxygenase Activity Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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