Vitamin B2 (Riboflavin) Test
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Price
$95 per analysis
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Turnaround time 7 - 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
Samples are autoclaved in HCl to breakdown the matrix. Potassium permanganate is added to the filtrate to oxidize interfering fluorescent compounds. Sodium bisulfite is then added to decolorize residual potassium permanganate. The fluorescence intensity (in the 440-550 nm range) of the vitamin is then measured. Blank measurements to allow for interfering fluorescence are performed on samples where sodium hydrosulfite is added that quenches riboflavin's fluorescence. The net fluorescence is compared to a prepared standard curve.
- Acceptable matrices
Food and Food Ingredients
- Limit of quantitation
0.02 mg/100g to 0.1 mg/100g, depending on sample weight
- Equipment
Fluorescence Spectrophotometer, Auto-analyzer with fluorescence detection
- Method reference
AOAC 942.23, 970.65 and 981.15
- Reportable unit
mg/100gram
- Sample size requirements
10 g
- Information required by submitter
Please supply an estimate of expected riboflavin content and indicate the presence of iron in samples.
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
Common Vitamin B2 (Riboflavin) Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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