Vitamin B6 (Pyridoxine) Test
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Price
$125 per analysis
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Turnaround time 7 - 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
This is a microbiological assay employing the organism Saccharomyces uvarum to determine the quantity of vitamin B6 (pyridoxine, pyridoxal, and pyridoxpyridoxine) in food products and vitamin concentrates. The ground, homogenized sample is dissolved or dispersed in an acidic extraction solution. The mixture is autoclaved at 121 - 123 °C to extract vitamin B6. The pH of the extract is adjusted to 4.4 and diluted with a basal medium containing all required growth nutrients for the microorganism except vitamin B6. The mixture is inoculated with the microorganism and incubated for 22 hours at 30°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of vitamin B6. The result is expressed as vitamin B6 (pyridoxine). The method is semi-automated utilizing an automated pipettor system and an automated plate reader system.
- Acceptable matrices
This method is applicable to cereals, beverages, vitamin concentrates, and dairy products containing added vitamin B6 and/or other free forms of vitamin B6.
- Unacceptable matrices
Premixes, concentrates, supplements above 4%
- Limit of quantitation
0.02 mg/100g to 4000 mg/100 g
- Equipment
Automated pipettor system Automated plate reader system
- Method reference
AOAC 961.15, 985.32, 960.46
- Reportable unit
mg/100g
- Sample size requirements
10 g
- Information required by submitter
Please supply estimates
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. This method is not designed for use on vitamin concentrates.
Common Vitamin B6 (Pyridoxine) Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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